Some cable or satellite providers title this "Pilot".
The song titled A Bunch Of Bones was used in many commercials advertising the show's first premiere.
This would probably explain why A Bunch of Bones is one of the most well-noted songs from the series.
Chronologically, this is not the first episode of the series as Bubble Puppy appears in this one but was formally introduced in Bubble Puppy! .
When Avi hits a rock with his tricycle, he doesn't fall off right away, and just sits there for a second before falling. Some say Avi could be faking his own injury, but the x-rays showed that he most certainly fractured his tail. The pause may have been there for humor.
For some unknown reasons, Noggin did not air this episode until Memorial Day 2013.
The independent syndicated version episode entitled: "Sick Days", recycles the sick animals segment from this episode.
This episode was the basis of Jeopardy! question: "This episode of Bubble Guppies, depicts a young blue fish who suffers an injury."
NCIS stars Mark Harmon, Cote de Pablo, & Pauley Perrette told the creators this episode was their favorite of the whole series.