- In Season 1 of the show, the order of the Guppies are: Oona, Nonny, Deema, Gil, Molly, and Goby. In Season 2 onward, the order is: Nonny, Oona, Deema, Goby, Gil, and Molly, the reverse order of how they introduce themselves in the theme song.
- During Season 1, Nonny was seen smiling, but during season 2 he isn't smiling.
- In Season 2, this song now plays before the dance segment.
- In Good Morning, Mr. Grumpfish! the instrumental of this song plays instead of the characters singing it.
- Usually, if the Guppies are wearing costumes before it's time to line up, they are no longer wearing them during the song. In the episode Gobble Gobble Guppies!, things are different: the Guppies wear sweaters and sleeveless jackets during the song.
- In A Very Guppy Christmas!, Mr. Grouper and the kids dress up in winter outfits.
- In Guppy Style, Princess Demanda sang a variation of this song while scolding her dragon, Destiny.
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